Tutorial

 

MiSNPDb can be accessed at: http://webtom.cabgrid.res.in/mangosnps/ 
MiSNPDb webserver comprise of six tabs viz. Home, Browse, Search, Analysis, Tutorial and Team.

Home Tab
Home tab includes description, characteristics and heterozygosity of Mango and usage of database.


 

Browse Tab

 


Browse Tab contains the list of the varieties of mango included in the database. User has to select a variety based on his requirements and click on search button.


On selecting a particular variety user is directed to SNPs in that variety along with the descriptions like Depth, SNP Position, Major and Minor allele and the total Number of SNPs.

 


On clicking on Details tab in the results, user are directed to details of that particular SNP which includes Sample name, Locus, SNP Position , Major and Minor allele and the Sequence containing the SNP and counts and percentage of the haplotype.

 


Search Tab
On selecting the Search tab User can search on the basis of different search parameters like variety name, haplotype, depth and All SNPs or only the common SNPs.
Example 1. Suppose user want to analyse All SNPs from Afeam variety with Depth from 2 to 5 containing the haplotype TATAAT. We enter the required fields, select the radio button of All SNPs and click on submit.


On clicking on submit button results for the search are displayed as SNP ID, Locus ID Haplotype, Stack Depth, Position, Major and Minor allele.


On clicking on the Details tab in the results, the user get the details of the particular SNP like the Sample name, Locus, Position, Major and Minor allele and the Sequence with the SNP shown in red along with percent and counts of the haplotype.


Search criteria
Example 2. Suppose we want analyze the Common SNPs from three samples Afeam, Alphan and Alphonso with stacks depth from 2 to 5. User enter the required fields, select the radio button Only Common SNPs and click submit

 
 

On clicking submit button the results are displayed which include the SNPs which are common in the three varieties and fulfill the search criteria. On scrolling over a column of the results, description of the column is shown eg. Mango Variety.

 


On selecting a particular radio button of the SNP ID we are directed to the results of only that particular SNP ID across the samples.

 

On selecting a particular radio button of the SNP ID we are directed to the results of only that particular SNP ID across the samples.

 


On selecting the particular radio button of the Locus ID we are directed to the results of that locus across the samples.


On selecting the particular radio button of the Locus ID we are directed to the results of that locus across the samples.


Tutorial Tab contains the tutorial of this database.

 

 Tutorial Tab contains the tutorial of this database.