This book contains information on dnadb, the sequence submission database, an annotated collection of all publicly available DNA sequences.This book will help you begin the dnadb submission process. It is arranged as a topical reference, so you do not have to read from beginning to end to find specific pieces of information.Provide information that will address common dnadb submission questions.

• First time user has to go through registration details through the Sequence Submission Portal Login System with the information like, name, telephone, address, and e-mail address of the submitter.

• A basic information has to be provided by the submitter about the Sequence authors like, First, Middle and Last name and a working title for a corresponding reference paper mainly, Unpublished (Submitted) - an article "submitted"for publication or "in preparation “Accepted for Publication (i.e. In-press) or Published".
• A sequence data in the FASTA format can be either cut-and-pasted as text into the given space. A source information viz. name(s) of the organism(s) from which the sequence data were isolated and any other related description details like, Molecular type (indicate the type of molecule present in the cell), Topology (indicate the shape of the DNA molecule. It can be Linear, Circular or Supercoiled), Genetic completeness (Show the partial or complete form of DNA) and Source organism name (an appropriate scientific name for the source organism is crucial for proper taxonomic assignment and genetic code use for conceptual translation of coding regions).
• Full sequence features has to be entered into the portal space viz. CDS, gene, rRNA, tRNA, with nucleotide intervals and product names.

Features of the portal:

Options for Submission

How to use this portal:

Requirements for Sequence Submissions through dnadb

• Contact Information
• Name, address, phone number, fax number and email address of the submitter must be entered when registering and submitting for the first time
• Subsequent dnadb submissions will retain this information and display it once the submitter logs in
• Release date information
• Immediately after it is processed at NCBI OR
• On a date the submitter specifies
• Reference information
• Sequence authors: names of the researchers who are credited with the sequence
• Publication information: Unpublished, In-Press, or Published; and applicable citation information (paper's title, authors, journal title, volume, issue, year, pages)
• Sequencing Technology
• This information is required if you are submitting over 500 sequences or if your sequences were generated using next-generation sequencing technology.
• What methods were used to obtain these sequences?
• Submission Category and Type
• Original sequencing or Third Party Annotation
• Nucleotide sequence(s)
• Sequence data in the FASTA format can be either cut-and-pasted as text or uploaded from the local folder.
• Sequences must be at least 200 nucleotides long.
• Molecule type: what was sequenced? (genomic DNA, mRNA, genomic RNA, cRNA, etc)
• Topology: linear or circular (circular must be complete, such as a complete plasmid)
• Organism name, applicable source modifiers, location
• Source organism name (an appropriate scientific name for the source organism is crucial for proper taxonomic assignment and genetic code use for conceptual translation of coding regions).
• Most complete name for any synthetic vector (for example: Cloning vector pAB234, Transfer vector p789Abc)
• Source modifiers include: strain, clone, isolate, specimen-voucher, isolation-source, country
• Location: organelle (mitochondrion, chloroplast, etc); map and/or chromosome
• Features of the sequence
• use input forms to add all applicable features (for example: CDS, gene, rRNA, tRNA, microsatellite, exon, intron)